Protein-coding gene in the species Homo sapiens
Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene .[5] [6] [7] It is the homolog of the Xenopus protein XMAP215 [8] and is also known as ch-Tog .
It has at least two distinct roles in spindle formation: it protects kinetochore microtubules from depolymerization by MCAK (KIF2C ), and ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity.[9]
Interactions
CKAP5 has been shown to interact with TACC1 .[10] [11]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000175216 – Ensembl , May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000040549 – Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Nagase T, Miyajima N, Tanaka A, Sazuka T, Seki N, Sato S, Tabata S, Ishikawa K, Kawarabayasi Y, Kotani H (Jul 1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1" . DNA Res . 2 (1): 37–43. doi :10.1093/dnares/2.1.37 . PMID 7788527 .
^ Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996). "Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors" . Eur. J. Biochem . 234 (2): 406–13. doi :10.1111/j.1432-1033.1995.406_b.x . PMID 8536682 .
^ "Entrez Gene: CKAP5 cytoskeleton associated protein 5" .
^ Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly" . Mol. Biol. Cell . 15 (4): 1580–90. doi :10.1091/mbc.E03-07-0544 . PMC 379257 . PMID 14718566 .
^ Barr AR, Gergely F (2008). "MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics" . Mol. Cell. Biol . 28 (23): 7199–211. doi :10.1128/MCB.01040-08 . PMC 2593372 . PMID 18809577 .
^ Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (Nov 2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene . 22 (50): 8102–16. doi :10.1038/sj.onc.1206972 . PMID 14603251 . S2CID 10536264 .
^ Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (Apr 2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells" . Biochem. J . 363 (Pt 1): 195–200. doi :10.1042/0264-6021:3630195 . PMC 1222467 . PMID 11903063 .
External links
Further reading
Basto R, Gergely F, Draviam VM, Ohkura H, Liley K, Raff JW (2007). "Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans" . J. Cell Sci . 120 (Pt 7): 1278–87. doi :10.1242/jcs.000604 . PMID 17376965 .
Kosturko LD, Maggipinto MJ, D'Sa C, Carson JH, Barbarese E (2005). "The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2" . Mol. Biol. Cell . 16 (4): 1938–47. doi :10.1091/mbc.E04-08-0709 . PMC 1073673 . PMID 15703215 .
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins" . Proc. Natl. Acad. Sci. U.S.A . 101 (33): 12130–5. Bibcode :2004PNAS..10112130B . doi :10.1073/pnas.0404720101 . PMC 514446 . PMID 15302935 .
Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly" . Mol. Biol. Cell . 15 (4): 1580–90. doi :10.1091/mbc.E03-07-0544 . PMC 379257 . PMID 14718566 .
Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene . 22 (50): 8102–16. doi :10.1038/sj.onc.1206972 . PMID 14603251 . S2CID 10536264 .
Gergely F, Draviam VM, Raff JW (2003). "The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells" . Genes Dev . 17 (3): 336–41. doi :10.1101/gad.245603 . PMC 195983 . PMID 12569123 .
Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs" . Genome Res . 12 (11): 1773–84. doi :10.1101/gr.406902 . PMC 187542 . PMID 12421765 .
Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells" . Biochem. J . 363 (Pt 1): 195–200. doi :10.1042/0264-6021:3630195 . PMC 1222467 . PMID 11903063 .
Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery" . Genome Res . 6 (9): 791–806. doi :10.1101/gr.6.9.791 . PMID 8889548 .
Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells" . PLOS ONE . 7 (12): e51442. Bibcode :2012PLoSO...751442N . doi :10.1371/journal.pone.0051442 . PMC 3520847 . PMID 23251535 .
Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels". Nat. Cell Biol . 15 (6): 688–93. doi :10.1038/ncb2744 . PMID 23666085 . S2CID 3025200 .